Supplementary MaterialsAdditional file 1: Figure S1

Supplementary MaterialsAdditional file 1: Figure S1. or colchicine (c and d), at 2?h and 24?h coCculture time. Scale bars, 10?m. 12964_2019_472_MOESM2_ESM.pdf (1.8M) GUID:?D2FBBCF1-4ABF-4584-B420-B8C179395096 Additional file 3: Figure S3. Analysis of mitochondrial morphology. a Representative image of mitochondria networks labeled with MitoT Deep Red in untreated fibroblast. b TIC10 isomer Segmentation of white boxed area in a. c Colour maps of aspect ratio (AR), circularity and roundness as in b. d Mitochondria exchange between unirradiated (MitoT Deep Red) and 6CGy irradiated (MitoT Green) fibroblasts, under untreated, taxol and colchicine conditions. e Single cell analyses of mitochondria shapes of MitoT Green from acceptor cells in d. 12964_2019_472_MOESM3_ESM.pdf (1012K) GUID:?5B833346-5BD6-4A49-B615-E25F0407C2E9 Additional file 4: Figure S4. p105 a Mitochondria exchange between DNACdamaged and healthy fibroblasts. Corresponding to Fig. ?Fig.1e.1e. The absolute values of average aspect ratios (avg. AR). b Comparison of indicated conditions to 2Ch control. Results represent average ARCvalues of 30 cells SD (twoCsided tCtest; ns, not significant, *P?P?P?n?=?300 (at first time point) to 5000 (at last time point). g Reduction of 53BP1 foci number in acceptor cells depends on ratio of donorCtoCacceptor cell numbers. Results represent mean??SD (twoCsided tCtest; ns, not significant, *P?P?P?TIC10 isomer monitor G1 (red) and G2 (green) phases. Transfected cells were exposed to 6?Gy xCray and subsequently plated for either monoC or coCculture together with untreated MiaPaCaC2 cells. After 24?h, 53BP1 foci were determined. In overlay pictures, the first number indicates the ratio of G1 to G2 fluorescence intensity and the second number indicates the foci number in each nucleus. Scale bars, 10?m. c Storyline of fluorescence strength ideals for G2 vs G1 in each analysed cell. Size of group radius represents amount of foci. d Storyline of G1 to G2 percentage over amount of 53BP1 foci. Pubs represent solitary cell. e Percentage of cell amounts in various cell cycle stages. 12964_2019_472_MOESM8_ESM.pdf (223K) GUID:?50C2F989-C9B5-498F-96CA-E7DB647C413D Extra document 9: Figure S9. DSB restoration is considerably influenced by acceptor cell/donor cell relationships within an ATM activityCdependent way. a ATMwt fibroblasts had been treated with DNACPK inhibitor (DNACPK i) and 6?Gy xCrays. PhosphoCDNA PK S2056 foci had been established at 1?h post irradiation by immunofluorescence microscopy. DMSO utilized.