Supplementary MaterialsFIGURE S1: Systemins from Solanaceous species induced-resistance assays against in Arabidopsis plants

Supplementary MaterialsFIGURE S1: Systemins from Solanaceous species induced-resistance assays against in Arabidopsis plants. least 3 x with similar outcomes. Picture_1.TIF (1.4M) GUID:?6E3B3541-973A-49A9-BF29-D07A4E442D62 FIGURE S2: Antimicrobial peptides from radish induced-resistance assays against in Arabidopsis plant life. Infection amounts 5 times after inoculation quantified by an illness ranking in trypan blue stained leaves, measured as a percentage of the infected leaf surface. Arabidopsis Col-0 plants were treated with increasing concentrations of AFP1 and AFP2 (0.1, 1, 10, and 20 nM) 24 h before contamination with 6 l droplets of 5 10E3 spores/ml of BMM. ?-amino butyric acid (BABA) at 1 ppm was used as a positive control. Colors mean % of diseased leaves in a level (0 = healthy leaves; 1 = leaves with less than 25% of diseased surface; 2 = leaves with 25C50%; 3 = leaves with 50C75% of the diseased surface, 4 = leaves with more than 75% of the surface diseased). Different letters indicate statistically significant differences (ANOVA, Fishers Least Significant Difference (LSD) test; 0.05, = 6). The experiment had 6 plants per treatment and was repeated at least three times with similar results. Image_2.TIF (1.4M) GUID:?A56F8484-AB46-4E8B-BF4D-884AA652C9BA Physique S3: Soybean peptides induced-resistance assays against in Arabidopsis plants. Contamination levels 5 days after inoculation quantified by a disease rating in trypan blue stained leaves, measured as a percentage of the infected leaf surface. Arabidopsis Col-0 plants were treated with increasing concentrations of GmPep914 and GmPep890 (0.1, 1, 10, and 20 nM) 24 h before contamination with 1 l droplets of 5 103 spores/ml of BMM. Colors mean % of diseased leaves in a level (0 = healthy leaves; 1 = leaves with less than 25% of diseased surface; 2 = leaves with 25C50%; 3 = leaves with 50C75% of the diseased surface, 4 = leaves with more than 75% of the surface diseased). Different letters indicate statistically significant differences (ANOVA, Fishers Least Significant Difference (LSD) test; 0.05, = 6). The experiment had 6 plants per treatment and was repeated at least three times with similar results. Image_3.TIF (1.3M) GUID:?2CCEC805-0092-457A-9E25-F0C56DF00307 FIGURE S4: Induced- Resistance assays of Sys-P13AT17A in Arabidopsis and AtPep1 in tomato. Contamination levels of Arabidopsis Col-0 plants treated with 0.1 nM of truncated Systemin (Sys-P13AT17A) (A) and tomato wild-type plants treated with increasing concentrations of AtPep1 (0.1, 1, 10, and 20 nM) (B) 24 h before contamination. Contamination was quantified 5 days after inoculation with 1 l droplets of 5 103 spores/ml of BMM by a disease rating in trypan blue stained leaves, measured as a percentage of the infected leaf surface. Colors mean % of diseased leaves in a level PA-824 kinase activity assay (0 = healthy leaves; 1 = leaves with less than 25% of diseased surface; 2 = leaves with 25C50%; 3 = leaves with 50C75% of the diseased surface, 4 = leaves with more than 75% of the surface diseased). Different letters indicate statistically significant differences (ANOVA, Fishers Least Significant Difference (LSD) test; 0.05, = 6). The experiment had 6 plants per treatment and was repeated at least three times with similar results. Image_4.TIF (582K) GUID:?E62BAECC-374C-4428-AEC0-BBDEE9991EC1 FIGURE S5: Peptides measured by HPLC-MS Infection quantification by measuring fungal biomass. A ratio of relative to was calculated after performing a qPCR from gDNA of Arabidopsis infected plant samples 48 h after pathogen inoculation in watered plants and plants treated with 0.1 nM systemin 24 h before inoculation of Bars represent mean PA-824 kinase activity assay standard error (SD), = 6. Asterisks imply statistical significant differences; 0.05, = 6. Image_7.TIF (21K) GUID:?E4D10C36-A761-4655-9731-DD03EA28C708 FIGURE S8: ROS production areas in response to Systemin and PAMP challenge. H2O2 production was measured during 1 h in leaf disks after elicitation with Systemin at different concentrations and with 100 nM flg22 in DCN leaf disks that were pre-treated for 24 h with different concentrations of Systemin. Luminescence was expressed in Relative Luminescence Units. Bars represent means of peak areas PA-824 kinase activity assay standard error (SD), = 8. Different words represent significant differences statistically. (ANOVA, Fishers Least FACTOR (LSD) check; 0.05, = 8). Picture_8.TIF (37K) GUID:?988B4421-1113-4F7D-8F68-DD5C2582E5D2 FIGURE S9: Sys-IR assays in the mutant. Col-0 and plant life had been challenged with 1 l droplets of 5 10E3 spores/ml of BMM 24 h after treatment with 0.1 nM Systemin. Infections levels had been quantified 5 times after inoculation by an illness.