Support structures for dermal regeneration are composed of biodegradable and bioresorbable polymers, animal skin or tendons, or are bacteria products. toxicity were analyzed after 7 days. The cultured cells showed uniform growth and morphological characteristics of undifferentiated MSCs, which demonstrated that MSCs successfully adapted to the culture conditions established by collagen scaffolds with or without HA. This demonstrates that such scaffolds are promising for applications to tissue regeneration. bFGF significantly increased the proliferative rate of MSCs by 63% when compared to groups without the addition of the growth factor. However, the addition of bFGF becomes limiting, since it has an inhibitory effect at high concentrations in culture medium. expansion potential (1-3), and ability to differentiate into mesenchymal (4), ectodermal (5,6), and endodermal (7) cells. These cells have the capacity to differentiate into bone, cartilage, fat tissue, tendon, muscle (8,9), mature hepatocytes, epithelial cells of the skin and intestinal tract, and may improve heart function after myocardial infarction (10-12). Numerous tissue engineering strategies are available today for the regeneration of wounded skin. The use of collagen membrane gels as biomaterials in plastic surgery is one of them and has greatly improved in recent decades. This has ultimately led to an increased interest in developing biodegradable and biocompatible materials for tissue regeneration (13-15). The increasing importance of collagen in the area of biomaterials is due to its great abundance in the animal kingdom, the ease in obtaining it, and its positive physical and physicochemical properties for specific applications in biomaterials. Hyaluronic acid (HA) has a high ability to absorb water, which influences several cellular functions such as migration, adhesion, and cell proliferation (16,17). Furthermore, HA is a major component of the extracellular matrix of mammals. Therefore, its application to the field of biomaterials is promising, currently being used as bio-implants for the treatment of voice disorders (18), and as an injectable gel in plastic surgery for the management of facial aging (16,19). Among growth factors, the fibroblast growth factor (FGF) family (22 family members) is of great importance in the process of tissue regeneration. Most members of this family have a wide range of mitogenic action and can stimulate the proliferation of cells of ectodermal, mesodermal, and endodermal origin (20). Treatment based on biomaterials is still controversial due to its low efficiency. However, it is believed that the combined use of biodegradable membranes with stem cell Gestodene therapy may generate promising results for patients submitted to unsuccessful conventional treatments (21). Gestodene Thus, compounds made Rabbit Polyclonal to ADCK1 from the combination of collagen, HA, and growth factors for the application of Gestodene injectable biomaterials or in the form of membranes have been developed to attract cells, serving as a scaffold for tissue growth (14). In this study, bone marrow-derived canine mesenchymal stromal cells (MSCs) were applied to an anionic collagen membrane with and without HA (22). The membrane served as support for adhesion and growth of the cells to develop a cellularized graft and to assess the effect of basic FGF (bFGF) on the proliferation of cultured MSCs. Material and Methods Preparation of biocomposites from native and anionic collagen Anionic collagen (Coll) was developed by the Department of Biomaterials, USP, S?o Carlos, SP, Brazil (22). HA, obtained for the manufacture of membranes, was extracted from chicken ridges using a method developed by the team of Biochemistry and Biomaterials from the S?o Carlos Institute of Chemistry (USP), which is a proprietary process (patent Gestodene pending). For the preparation of the composites, 10:1 and 100:1 ratios (Coll:HA) were used. After.