The specificity and sensitivity of immunoblot analysis were 83

The specificity and sensitivity of immunoblot analysis were 83.3-91.7% and 100%, [10] respectively. SAG Open in another window Fig. the 118 non-traumatic SAH sufferers for whom cerebral immunoblot and angiogram data had been obtainable, 80 (67.8%) sufferers had A-SAH, whereas 38 (32.2%) had NA-SAH. General, 23.7% were positive for particular antibodies against 21- and/or 24-kDa antigen. No significant distinctions were within the positive price of particular antibodies against in both groupings (is normally one possible reason behind SAH in Thailand [8], but scientific data over the regularity of gnathostomiasis in SAH patients is still limited even in areas where gnathostomiasis is usually endemic. The diagnosis of neurognathostomiasis by detection of larva is extremely rare [9]. It is, therefore, worthwhile to use immunoblot analyses to diagnose neurognathostomiasis. In this study, we examined anti-antibody-positive rate in sera of SAG non-traumatic SAH patients in Thailand to elucidate the significance of infection as a cause of SAH. We enrolled non-traumatic SAH patients diagnosed at the Srinagarind Hospital, Khon Kaen University, Khon Kaen, Thailand between January 2011 and January 2013. SAH was verified by non-contrast CT or MR imaging of the brain. The inclusion criteria included non-traumatic SAH patients for whom 1) results of CT brain imaging (Fig. 1) or MR imaging of the brain were available, 2) results of CT angiography (CTA) or MR angiography (MRA) or 3-dimensional digital subtraction cerebral angiography (DSA) were available (Fig. 2) to identify any intracranial aneurysm that might be present (Fig. 3), and 3) results of immunoblot analysis for antibodies against 21-or 24-kDa antigen band of were avaiable. The sensitivity and specificity of immunoblot analysis were 83.3-91.7% and 100%, respectively [10]. Open in a separate windows Fig. 1 Non-contrast CT axial view showing hyperdense area of subarachnoid hemorrhage (SAH) along the interhemispheric fissure. Open in a separate windows Fig. 2 Three-dimensional digital subtraction cerebral angiography (DSA) showing the normal posterior circulation artery, vertebral artery, basilar artery, posterior cerebral artery, and superior cerebellar artery. Open in a separate windows Fig. 3 Three-dimensional digital subtraction cerebral angiography (DSA), lateral view of internal carotid artery and anterior cerebral artery showing subarachnoid hemorrhage SAG from aneurysm at anterior communicating artery. All eligible patients were categorized into 2 groups; aneurysmal SAH (A-SAH) and non-aneurysmal SAH (NA-SAH). The former group included those SAH patients with intracranial aneurysm detected by CTA and/or MRA and/or DSA. The technique and procedures of DSA have been described elsewhere [11]. Patients with no abnormal cerebral vessels according to DSA were included in the NA-SAH group. Sera of Rabbit Polyclonal to iNOS members of both groups were examined for the presence of anti-antibody and levels of seropositivity compared between the groups. The study protocol was approved by the Khon Kaen University Ethics Committee for Human Research (“type”:”entrez-nucleotide”,”attrs”:”text”:”HE551056″,”term_id”:”288736597″,”term_text”:”HE551056″HE551056). During the study period, 118 patients met the criteria. The antibody positive rate in A-SAH group was SAG 26.2% (21/80) and that of NA-SAH group was 18.4% (7/38). Although the antibody-positive rate of A-SAH group was somewhat higher than in NA-SAH group, there was no statistically significant difference between them (by immunoblotting Open in a separate windows aBy one-way ANOVA. bBy Fisher’s Exact test. In this study, an overall antibody-positive rate in non-traumatic SAH was 23.7%, but the antibody-positive rate between A-SAH and NA-SAH groups was not significantly different from each other, suggesting that infection is a risk factor for SAH but not associated with particular background status of SAH. The samples were selected not just from suspected gnathostomiasis patients but also from overall SAH patients. This is the seroprevalence value of antibodies in SAH patients in Thailand. Since the sample size of this study is usually too small, further accumulation of the samples is necessary to draw any solid conclusion. Gnathostomiasis patients with neurological manifestations do not always have cutaneous intermittent migratory swelling or radicular pain [7,12-14]. Therefore, physicians working in gnathostomiasis endemic areas should aware that gnathostomiasis can be a cause of SAH. In this study, IgG antibody detection using immunoblotting was employed to detect contamination, so that there is a risk of surpassing antibody-negative or IgM-antibody positive acute stage cases. However, this possibility is rather unlikely because most of neurognathostomiasis patients in Thailand have infections for years before development of neurological complications [7]. In conclusion, the detection rate of antibodies in non-traumatic SAH was 23.7%. The rate was somewhat higher in A-SAH than in NA-SAH group. Other history findings should be included for supportive evaluation. Whether such a detection rate is seen in other countries or in other diseases should be clarified in a comparative study. ACKNOWLEDGMENTS This research was.