The cockerels raised in the next-door barn on the same premises were not affected

The cockerels raised in the next-door barn on the same premises were not affected. study. This group-specific ELISA is therefore a sensitive and practical way to monitor FAdV antibodies in commercial flocks. None of the 1-d-old chicks tested were positive by PCR, nor was FAdV isolated UNC1215 from the same tissues, indicating an absence of transmission of infectious virus to the progeny. The lack of virus production and transmission could be due to the presence of high antibody titers in the layers. == Rsum == Laugmentation du nombre de cas cliniques dhpatite corps dinclusions (IBH) associs avec ladnovirus aviaire (FAdV) est une proccupation grandissante dans diffrentes parties du monde, incluant le Canada. Suite un pisode dIBH chez des poulettes de 10 jours dge dans un troupeau doiseaux reproducteurs, un suivi srologique du troupeau a t effectu de lge de 8 semaines jusqu 46 semaines utilisant un test de prcipitation en agar (AGPT) offert par des laboratoires de diagnostic et une preuve immunoenzymatique (ELISA) spcifique au groupe FAdV dveloppe antrieurement. De plus, la progniture a t teste pour vrifier une possible transmission verticale lorsque le troupeau reproducteur approcha le pic de production dufs, en effectuant une preuve disolement viral et une raction damplification en chane par UNC1215 la polymrase (PCR) sur les organes cibles doiseaux g de 1 jour. Tout comme lors dtudes antrieures comparant les deux preuves, lELISA tait plus sensible que lAGPT. Avec lpreuve ELISA quelques oiseaux ont montr une raction faiblement positive 8 semaines dge, et tous les oiseaux Rabbit Polyclonal to CYB5R3 prsentaient des rsultats fortement positifs partir de 12 semaines dge jusqu la fin de ltude. Lpreuve ELISA spcifique de groupe est ainsi sensible et pratique pour surveiller les anticorps anti-FAdV dans les troupeaux commerciaux. Aucun des poussins de 1 jour tests ne sest avr positif par PCR de mme que pour lisolement de FAdV partir des mmes tissus, indiquant ainsi labsence de transmission du virus infectieux la progniture. Labsence de production virale et de transmission pourrait tre due la UNC1215 prsence de titres danticorps levs chez les pondeuses. (Traduit par Docteur Serge Messier) == Introduction == Inclusion body hepatitis (IBH) is usually seen in chickens 3 to 6 wk old. The 1st sign is often a sudden increase in mortality rate in the flock, reaching sometimes 5% to 10%; the rate then returns to normal after 5 to 7 d. The mortality pattern, the age of the flock, and the gross and histopathological findings post mortem are characteristic for the disease, and fowl adenoviruses (FAdVs) can be isolated from the affected birds (14). Immunosuppressive agents such as infectious bursal disease virus and chicken infectious anemia virus are thought to predispose chickens to IBH (57). Fowl adenoviruses are ubiquitous and often of low virulence. To date, 11 serotypes in the genusAviadenovirusof the familyAdenoviridaehave been recognized. All FAdVs share a group-specific antigen. Certain FAdVs are more pathogenic than others: serotype 4 has been involved in hydropericardium syndrome, and serotype 8 has been implicated in peracute IBH (1,2,5,8). Recently the increased number of FAdV-associated outbreaks of IBH has renewed interest in investigating these viruses, disease pathogenesis, and diagnostic options (912). Serologic surveys of adenovirus infections have been performed at a certain age of chickens and representing a given geographic area. Fowl-adenovirus-specific antibodies are commonly found in breeder and layer flocks, and exposure to multiple serotypes is documented (1316). The agar gel precipitation test (AGPT) is a widely used serologic test for the detection of FAdV antibodies, since it is fast and economical. However, it has several disadvantages, such as lack of sensitivity (16), and the detection of infection might be delayed in fowl since precipitins may develop only after a 2nd exposure to FAdV (17). The AGPT has a satisfactory sensitivity when performed on serum from commercial flocks that are often exposed to multiple adenovirus serotypes, but it is less sensitive in detecting adenovirus infections in specific-pathogen-free (SPF) flocks (4). The sensitivity of AGPT can be increased by using antigen pooled from different FAdV serotypes.