The study was conducted using CD-1 female mice housed 5 per cage

The study was conducted using CD-1 female mice housed 5 per cage. varying degrees of compassion fatigue, we formed an internal team dedicated to addressing compassion fatigue and improving compassion satisfaction. The name of the team is People Advocating Wellness and Support (PAWS). The mission is to be a cross-function team to raise awareness, educate, and stimulate open conversation about compassion fatigue and compassion satisfaction within our organization. The team will work to support and identify needs within our organization that can positively influence and support those people experiencing compassion fatigue. To help steer the direction of the team and identify needs of our people, we conducted a needs assessment in partnership with an external consultant. Our team has used the needs assessment to launch initiatives that help focus on our core ACE principle: awareness, communication, and education around compassion fatigue and compassion satisfaction. In the long term, we hope to positively support and improve the lives of our employees through PAWS. P2 Assessment of Microchip Placement Methods in Immunocompromised Mice A Hernandez*1, P Torres2, P Collier2, M Campagna1 1Comparative Medicine and Quality, The Jackson Laboratory, Sacramento, CA; 2The Jackson Laboratory, Sacramento, CA Microchipping has become an increasingly popular means of identification in laboratory mice. Compared to other identification methods, such as ear tags, microchips are less likely to cause irritation, fall out, or be misread. Microchipped mice can easily be scanned, and any associated data can be linked directly to their microchip numbers. General anesthesia is often considered to be the most controlled method of restraint for microchip implantation, but NBQX other options exist. In our facility, physical restraint devices are commonly used without complication in immunocompetent mice. However, special considerations are often needed when translating methods to immunocompromised animals, such as usage of more stringent practices. Therefore, a study was conducted to assess complication rates in immunocompromised mice implanted with microchips using either physical or chemical restraint, and with or without surgical skin preparation. We hypothesized that complication rates would be similar between these different techniques. NSG (NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ) and NSG-SGM3 [NOD.Cg-Prkdcscid Il2rgtm1Wjl Tg(CMV-IL3,CSF2,KITLG)1Eav/MloySzJ] mice were divided into 4 groups (n=20 per NAV2 group). One group each of NSG and NSG-SGM3 mice were microchipped using a physical restraint device, and 2 groups of NSG-SGM3 mice NBQX were microchipped under isoflurane anesthesia. One anesthetic group was microchipped with a surgical skin preparation and the other was not. Mice were monitored for weight loss, signs of infection, and complications, such as inflammation, barbering, or microchip loss. After 2-8 wk, mice were euthanized for gross pathology, subcutaneous culture, and histology. The restraint device method was then similarly validated for groups (n=20) of nude (J:NU) and humanized (NSG and NSG-SGM3) mice. Pathological assessment did not reveal a NBQX significant difference in inflammation ( 0.999) between groups, and all culture results were negative. Weights postimplantation did not vary significantly between groups ( 0.06). The only noteworthy complication was occasional microchip loss immediately following implantation using the physical restraint device, with no persisting negative effects. We concluded that all of the assessed microchipping methods were acceptable for use on immunocompromised mice. P3 Dominance Status Has No Effect on Data Produced by SIV-infected Macaque ( 0.0001), indicating that tryptophan is being shunted away from serotonin and melatonin production in socially housed animals following SIV infection. This decrease in the proinflammatory metabolite kynurenine is consistent with the blunted immune response to SIV that we previously observed in singly NBQX housed animals. These data indicate that both the immune response in the CNS and the concentration of neurotransmitters are altered by social housing, and thus that single housing of SIV-infected animals has the potential to affect the translational value of data produced by the model. P15 Development of a Rabbit Enrichment Program and Contribution to a Culture of Transparency and Care T Worlds*2, F De La Garza2, K Kelly2, CZ Cannon1, M Jernigan2, G Jernigan2 1Becton Dickinson, Research Triangle Park, NC; 2Charles River Laboratories, Research Triangle Park, NC Rabbit models are used in the biomedical industry for good laboratory practice (GLP) NBQX safety and toxicity testing, antibody production, and preclinical studies. Rabbits are a.